Credits: 1 (1-0-0)
Prerequisites: EC 75 and SBL100 or Equivalent
Description
Introduction to mouse genetics – genotypes, genetic backgrounds and methods; Conventional methods of gene targeting and transgenics – gene delivery into embryonic stem cells or zygotes, gene-traps, breeding strategies; Establishment of cell culture models from mice – Fibroblasts and macrophage cultures, rescue strategies using retro/lentiviral transduction; Gene targeting in cells using CRISPR/ Cas9 - mutations, activation and suppression; In vivo conditional knockout strategies using Cre/lox and Flippase/FRT system; Bone- marrow chimeras; Animal models of human diseases.
Prerequisite Tree
flowchart TD
SBV894-699[SBV894]
SBV894-699 --> SBL100-699[SBL100]
SBV894-699 --> EC75-699[EC75]
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