Credits: 4 (3-0-2)
Description
Characteristic of animal cell, metabolism, regulation and nutritional requirements; Kinetics of cell growth and product formation and effect of shear force; Product and substrate transport; Perfusion bioreactors, Hollow fiber bioreactor, Operational strategies and integrated approach; Micro and macro carrier culture; Hybridoma technology; Genetic engineering in animal cell culture; Scale-up and large scale operation; Case studies.
Laboratory: Cell culture in static phase (T-flask), quantification of cell growth, monolayer culture, determination of critical shear stress, micro carrier, Cell viability assay. Case studies to understand growth kinetics and product kinetics in actul cell culture system.